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Western blot
After gel electrophoresis, transfer protein to membrane and then using antibody to detect protein expression.
Reference:
Pubmed  
Graph Resource
Luciferase reporter assay
The method can help to detect whether the miRNA is binding to mRNA or not.
Reference:
Pubmed  
Graph Resource
GFP reporter assay
Using green fluorescent protein to detect whether the mRNA is effect by miRNA and then change the protein expression.
Reference:
Pubmed  
Graph Resource
RT-PCR
Reverse transcriptase-PCR, the technique using reverse transcriptase transforms mRNA into cDNA. And then using PCR to amplify to detect the mRNA expression.
Reference:
Pebmed  
Graph Resource
pSILAC
Pulsed SILAC (stable-isotope labeling by amino acids in cultured cells), the method can detect protein abundance.
Reference:
Pubmed
Microarray
It is a high-throughput method to detect differentially expression mRNAs by the probe and sample hybridization.
Reference:
Pubmed  
Graph Resource
Branched DNA probe assay
The assay can detect and RNA or DNA target and quantified the target sample.
Reference: Pubmed
Northern blot
After gel electrophoresis, transform RNA onto membrane and using labeled probe to detect RNA expression.
Reference: Pubmed
TarBase (Papadopoulos GL et al., Nucleic Acids Res. 2009)
miRecords (Xiao F et al., Nucleic Acids Res. 2009)
miR2Disease (Jiang Q. et al, Nucleic Acids Res. 2009)
miRNAMap (Hsu SD et al., Nucleic Acids Res. 2008)
miRBase (Griffiths-Jones S et al., Nucleic Acids Res. 2008)
TargetScanS (Benjamin P. Lewis et al, Cell 2005)
microRNA.org (Betel D et al, Nucleic Acids Res. 2008)
miRDB (Xiaowei Wang, RNA 2008)
miRGator (Seungyoon Nam et al, Nucleic Acids Res. 2008)
miRGen (Panagiotis Alexiou et al, Nucleic Acids Res. 2009)
PicTar (Azra Krek et al, Nature Genetics 2005)
RNA22 (Ang, W.-L. et al, Cell 2006)
DIANA MicroT (Maragkakis; P. A. et al, BMC Bioinformatics 2009)
MicroInspector (Ventsislav R. et al, Nucleic Acids Res. 2005)
miRU (Yuanji Zhang, Nucleic Acids Rds. 2005)
TAPIR (Bonnet E. et al, Bioinformatics 2010)
RNAhybrid (Krüger J. et al, Nucleic Acids Res. 2006)
miRo (Laganà A. et al, Database 2009)
miRSel (Naeem H. et al, BMC Bioinformatics 2010)
MirOrtho (Gerlach D. et al, Nucleic Acids Res. 2009)
Patrocles (Hiard S. et al, Nucleic Acids Res. 2010)
miRNAtools
miRNA – Target Gene Prediction at EMBL (Stark A et al, Cell 2005)
miRBase (Kozomara, Ana et al., Nucleic Acids Res. 2019)
NCBI Entrez gene (Maglott, Donna et al., Nucleic Acids Res. 2011)
NCBI RefSeq (O'Leary, Nuala A et al., Nucleic Acids Res. 2016)
dbSNP (Sherry, S T et al., Nucleic Acids Res. 2001)
GWAS Catalog (Buniello, Annalisa et al., Nucleic Acids Res. 2019)
ClinVar (Landrum, Melissa J et al., Nucleic Acids Res. 2020)
COSMIC (Tate, John G et al., Nucleic Acids Res. 2019)
HMDD (Huang, Zhou et al., Nucleic Acids Res. 2019)
TransMir (Tong, Zhan et al., Nucleic Acids Res. 2019)
miRSponge (Wang, Peng et al., Database. 2015)
TissueAtlas (Ludwig, Nicole et al., Nucleic Acids Res. 2016)
EVmiRNA (Liu, Teng et al., Nucleic Acids Res. 2019)
Gene Expression Omnibus (GEO) (Barrett, Tanya et al., Nucleic Acids Res. 2013)
The Cancer Genome Atlas (TCGA) (Deng, Mario et al., BMC bioinformatics. 2016)
CMEP (Li, Jian-Rong et al, Bioinformatics. 2019)
MiREDiBase (Marceca, Gioacchino P et al., Scientific data. 2021)